New validationGreen Seal® GS-37 certification · April 21, 2026
/ Research

Every claim,
traced to its trial.

Bioferment's efficacy claims are not marketing. Each number on this page traces to a controlled trial run by the University of Florida or an independent lab — with full methodology, sample sizes, controls, and raw data on request.

Partners
5 labs
UF, Eurofins, PSL, Dell Tech, QIMA
Published trials
0
Across 4 product lines
Compliance
EPA 25(b)
Minimum-risk classification
Methodology
Open
Raw data on request
/ Trial data

Trial library.

CASE / 01HyphaWolf™ · PreHarvest + PostHarvestUF/IFAS Inoculation StudyPrincipal Investigator
Dr. Braham Dhillon100% GRAS-designated ingredients

Botrytis control on tomato & grape

Inoculation test run by Dr. Braham Dhillon at the University of Florida. Measured disease incidence against untreated control and a comparator sample across the crop types below. Worker-safe during active packhouse operations — zero restricted-entry interval. Dual-product platform: HyphaWolf PreHarvest for field coverage and HyphaWolf PostHarvest for cold storage.

MetricHyphaWolfReferenceREI
Botrytis on tomato65% ↓Untreated control0 hours
Botrytis on grape45% ↓Untreated control0 hours
Post-harvest, uninoculated fruitLower incidence than control & comparatorSample comparator—
PhytotoxicityNone observed——
Download full report (PDF) ↓See product page →
CASE / 02WaterWolf™UF/IFAS Fort Lauderdale REC · Sep–Nov 2024Principal Investigator
Dr. H. Dail Laughinghouse IVCo-investigator
David E. Berthold

100% cyanobacteria cell kill at 24 HAT

Two measurements, one result. Cell enumeration (Cells/mL) at 24 HAT shows complete kill at medium and maximum doses across low, medium, and high initial bloom densities, for both tested formulations. Chlorophyll-a reads 88.9% because residual pigment lingers in dead cells — cell count is the definitive measure of viable cells. Indirect comparison vs. GreenClean 5.0 is drawn against the Kinley-Baird et al. 2020 paper from the same Laughinghouse lab.

MetricWaterWolfMethodTime
Cyanobacteria cell kill (med + max dose)100%Cell count (Cells/mL)24 HAT
Chlorophyll-a reduction (max dose)88.9%Yéprémian (2017) chl-a24 HAT
Microcystin reduction (high dose / high cells)up to 65%MC concentration (µg/L)24 HAT
Species testedM. wesenbergii (direct); M. aeruginosa (per Lefler 2022)Morphological ID—
Download full report (PDF) ↓See product page →
CASE / 03BloomWolf™University of Florida · 48-hr Daphnia magnaPrincipal Investigator
Dr. H. Dail Laughinghouse IVCo-investigator
David E. BertholdEPA 25(b) Minimum Risk frameworkOECD Daphnia magna acute toxicity protocol

Phosphate capture + Daphnia safety

Phosphorus reduction tested at 5, 10, and 15 mg/L total product across pH 5–8. Paired with 48-hour Daphnia magna acute toxicity testing from 0.5% to 50% v/v to locate the no-effect boundary relative to the effective dose range.

MetricResultBenchmark
Phosphorus reduction25–45%Baseline P, pH 5–8
Effective dose range5–15 mg/LTotal product
No acute toxicity (Daphnia)Up to 2.5% v/v (~25,000 mg/L)Effect observed ≥ 5% v/v
Safety margin1,600×–5,000×Effective range vs. no-effect level
Download full report (PDF) ↓See product page →
CASE / 04MotherFerment™Dell Tech #37157 · Apr 8, 2026PSL Study #71721 · Jan 7–22, 2026Eurofins ECRLTX2023-0009 · ECRLTX2025-0170Green Seal® GS-37 · Apr 21, 2026

Efficacy, safety, preservation, stability, certification

Five independent validations on the current concentrated commercial cleaner (1:32 dilution). Dell Tech ASTM D4488-A5 for efficacy; Product Safety Labs OECD 425 for acute oral toxicity (Samantha Bloom, Study Director); Eurofins USP <51> for antimicrobial effectiveness (Olivia Wilde, PI); Eurofins CRL stability study (David Hernandez, PI); Green Seal GS-37 certification (Doug Gatlin, CEO, Green Seal).

MetricMotherFermentStandard · Lab
Cleaning efficacy at 1:3295.64%ASTM D4488-A5 · Dell Tech
Acute oral toxicity (LD50)> 5000 mg/kgOECD 425 · PSL (GLP)
Safer than table salt1.7×vs. NaCl LD50 ~3000 mg/kg
USP <51> Antimicrobial EffectivenessPASSED — all 5 organisms4.6–5.99 log → <1.71–2.71 · Eurofins
Shelf life1 year ambient8 wk / 40°C / 75% RH · Eurofins
Packaging — BPA · PFAS · PhthalatesAll Not DetectedHDPE bottles · QIMA
Green Seal GS-37 Certified SKUs93 variants × 3 sizes
Chemical products replaced40+Per facility audit
Download full report (PDF) ↓See product page →
CASE / 05MotherFerment Advanced Cleaner™ (AM5-1)Next-generation broad-spectrum antimicrobialEurofins CRL · ECRLTX2025-0492 · Nov 6, 2025ASTM E2315 Time-Kill Procedure

Time-kill pathogen reduction — 10 minutes

A new professional-grade SKU aimed at janitorial, healthcare, food service, schools, and childcare. Time-kill (ASTM E2315) against three ATCC reference organisms at 10-minute contact time ± 10 sec. Note: this is a distinct product from the original MotherFerment all-purpose cleaner — the 5.69 log E. coli result belongs to AM5-1, not the original formula.

OrganismLog reductionPercent
E. coli K12 NCTC 105385.69 log99.9998%
S. epidermidis ATCC 122284.67 log99.998%
P. fluorescens ATCC 135254.80 log99.998%
See the MotherFerment family →
/ Methodology

How Bioferment does research.

Lab partners
University of Florida IFAS (Fort Lauderdale REC, Plant Pathology), Eurofins Laboratories, Product Safety Labs, Dell Tech Labs, QIMA.
Controls
Every efficacy claim compares against both an untreated control and the best-in-class incumbent treatment.
Pre-registration
Protocols reviewed by PIs before execution. Raw data available on request for qualified technical reviewers.
Disclosure
Every on-site claim is traceable to a named lab, a named PI, a report ID, and a dated document. Headline numbers reflect the current concentrated commercial formulation; superseded values are available on request for historical transparency.
Named investigators
Dr. H. Dail Laughinghouse IV · David E. Berthold (UF phycology / HABs) · Dr. Braham Dhillon (UF plant pathology) · Samantha Bloom, BS (PSL Study Director) · David Hernandez, BS · Olivia Wilde, BA (Eurofins) · Jeremy Xu (QIMA) · Doug Gatlin (Green Seal CEO).
IP
Patent pending · Application #63/816,286 · Filed June 2, 2025 (docket 6437U.004 Provisional).
Cleaner Index
Bioferment maintains the Cleaner Index — an open methodology scoring 12 major cleaning brands on ingredient safety, environmental impact, and FTC compliance.
/ Technical briefs

Download the literature.

PDF · 18 pages · R&D

Biofermentation vs. plant-based surfactants

Monomer sourcing, fermentation yields, toxicology, supply chain.

Download →
PDF · 22 pages · Regulatory

EPA 25(b) registration dossier

Classification scope, ingredient disclosure, label claim substantiation.

Download →
PDF · 14 pages · Procurement

Facility cost-of-consolidation brief

Stocking, training, PPE, and incident-risk delta when replacing 20+ SKUs with MotherFerment.

Download →

Need raw data for your own review?

Qualified technical reviewers — procurement chemists, regulatory officers, university faculty — can request raw trial data, assay protocols, and unredacted methodology docs directly from the Bioferment science team.